 Nancy Denslow, Ph.D.
 Title: Adjunct Associate Research Fellow
Center of Innovative Research,
Professor, Physiological Sciences
University of Florida
Education :
Mary Washington College, Fredericksburg, VA, B.S. w/honors, Chemistry, 1966 Yale University, New Haven, CT, M.S., Biochemistry, 1967 University of Florida, Gainesville, FL, Ph.D, Biochemistry, 1975 University of Florida, Gainesville, FL, Post-Doc, Biochemistry, 1977-81
Positions and Employment 2008 - present: Professor, Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL
2004-2008: Associate Professor, Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL2001-2004: Scientist, Dept. of Biochem. and Mol. Biology, and ICBR, University of Florida.1993-2004: Scientific Director, Protein Chemistry and Molecular Biomarkers Core Facility, ICBR, University of Florida.1994-2001: Associate Scientist, Dept. of Biochem. and Molecular Biology, University of Florida.1988-1993: Technical Director, Protein Chemistry Core Facility, Interdisciplinary Center for Biotechnology Research, University of Florida1981-1993: Assistant Scientist, Dept. of Biochemistry and Molecular Biology, University of Florida1
1976-1977: Visiting Professor of Biochemistry, University of Ceara, Brazil
Other Experience and Professional Memberships
American Society for Biochemistry and Molecular Biology
Society of Toxicology
Society for Environmental Toxicology and Chemistry
Association of Biomedical Research Facilities, Executive Board member
Awards
2007 Pfizer Award for Research Excellence, College of Veterinary Medicine, University of Florda
Editorial Boards
Environmental Chemistry and Toxicology, 2002-present
Comparative Biochemistry and Physiology, Part D, Toxicogenomics, Associate Editor, 2004-present
Selected peer-reviewed publications (in chronological order).
(Publications selected from 120 peer reviewed publications)
Folmar, L.C., Denslow, N.D., Kroll, K., Orlando, E.F., Enblom, J., Marcino, J., Metcalfe, C., Guillette, L.J. Altered Serum Sex Steroids and Vitellogenin Induction in Walleye (Stizostedion vitreum) Collected Near a Metropolitan Sewage Treatment Plant. Arch. Environ. Contam. Toxicol, 40:392-398 (2001).
Denslow, N.D., Bowman, C.J., Ferguson, R.J., Lee, H.S., Hemmer, M.J. and Folmar, L.C. Induction of Gene Expression in Sheepshead Minnows Treated with 17-b-Estradiol, Diethylstilbestrol or Ethinylestradiol: The Use of mRNA Fingerprints as an Indicator of Gene Regulation. Gen.Comp. Endocrinol., 121:250-260 (2001).
Denslow, N.D., Lee, H.S., Bowman, C.J., Hemmer, M.J. and Folmar, L.C. Multiple Responses in Gene Expression in Fish Treated with Estrogen. Compar. Biochem. and Physiol. Part B 129: 277-282 (2001).
Bowman, C.J., Kroll, K.J., Gross, T.G., Denslow, N.D. Estradiol-induced gene expression in largemouth bass (Micropterus salmoides). Mol. Cell Endocrinol. 196:67-77 (2002).
Larkin, P., Sabo-Attwood, T., Kelso, J., and Denslow, N.D. Gene expression analysis of largemouth bass to estradiol, nonylphenol, and p,p’-DDE. Comparative Biochemistry and Physiology 133:543-557 (2002).
Larkin, P., Folmar, L.C., Hemmer, M.J., Poston, A.J., Denslow, N.D. Expression Profiling of Estrogenic Compounds using a Sheepshead Minnow cDNA Macroarray, EHP ToxicoGenomics, 111:29-36 (2003).
Sabo-Attwood, T., Kroll, K.J., and Denslow, N.D. Differential expression of estrogen receptor isotypes, alpha, beta and gamma by estradiol. Molecular and Cellular Endocrinology, 218:107-118 (2004).
Denslow ND, J. Kocerha, M.S. Sepulveda, T. Gross, and S.E. Holm, Gene Expression Fingerprints of Largemouth Bass (Micropterus salmoides) Exposed to Pulp and Paper Mill Effluents. Mutation Res. 552:19-34 (2004).
LaFleur Jr., G.J., D. Raldua, M. Fabra, O. Carnevali, N.D. Denslow, R.A. Wallace, J. Cerda, Fundulus Heteroclitus Yolk Proteins. I. Derivation from Parental Vitellogenins and Alternative Processing During Oocyte Maturation. Biol. Reprod. 73:815-824 (2005).
Denslow, N.D. and P. Larkin, P. Utilizing New Technologies for Bioindicator Research. Environmental Bioindicators, preview issue: 19-38 (2005).
Stevens S.M. Jr, A.Y. Chung, M.C. Chow, S.H. McClung, C.N. Strachan, A.C.Harmon, N.D. Denslow and L. Prokai L. Enhancement of phosphoprotein analysis using a fluorescent affinity tag and mass spectrometry. Rapid Commun Mass Spectrom. 19:2157-2162 (2005).
Garcia-Reyero, N., D.S. Barber, T.S. Gross, K.G. Johnson, M.S. Sepulveda, N.J. Szabo, N.D. Denslow. "Dietary exposure of largemouth bass to OCPs changes expression of genes important for reproduction." Aquat Toxicol. 78:358-369 (2006).
Ottens AK, G. Kitlen, W.E. Haskins, B.E. O’Steen, M.C. Liu, R.L. Hayes, N.D. Denslow, and K.K.W. Wang, A Quantitative Degredomics Approach To Biomarker Validation by nano-LC/MSMS, Analytical Chemistry, 77:4836-4845 (2005).
Ankley, GT., G.P. Daston, S.J. Degitz, N.D. Denslow, R.A. Hoke, S.W. Kennedy, A.L. Miracle, E.J. Perkins, J. Snape, D.E. Tillit, C.R. Tyler, D. Versteeg. Toxicogenomics in regulatory ecotoxicoloy. Environ Sci. Technol. 40: 4055-4065 (2006).
Barber DS, McNally AJ, Garcia-Reyero N, Denslow N.D. Exposure to p,p'-DDE or dieldrin during the reproductive season alters hepatic CYP expression in largemouth bass (Micropterus salmoides). Aquat Toxicol. 81:27-35 (2007).
Blum, J.L., Stuchal, L.D., James, M.O., Denslow, N.D. Stimulation of transactivation of the largemouth bass estrogen receptors alpha, beta-α and beta-β by methoxychlor and its mono-and bis-demethylated metabolites in HepG2 cells Journal Steroid Biochemistry and Molecular Biology, in press, 2007.
Sabo-Attwood, T., Blum, J.L., Kroll, K.J., Patel, V., Birkholz, D., Szabo, N.J., Fisher, S.Z., McKenna, R., Campbell-Thompson, M and Denslow, N.D. Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol. Journal Molecular Endocrinology, 39: 223-237 (2007).
Denslow, ND, Garcia-Reyero, N., and Barber, D.S. Fish ‘N’ chips: the use of microarrays for aquatic toxicology. Mol. BioSyst., 3: 172-177 (2007).
Wasdo, S.C., Barber, D.S., Denslow, N.D., Powers, K.W., Jorganes, M.P., Stevens Jr., S.M., Moudgil., B., and Roberts, S.M. Differential binding of serum proteins to nanoparticles. International Journal of Nanotechnology. 5:92-115 (2008).
Blum, J. L., Stuchal, L. D., James, M. O., Denslow, N. D. Stimulation of transactivation of the largemouth bass estrogen receptors alpha, beta-α and beta-β by methoxychlor and its mono-and bis-demethylated metabolites in HepG2 cells. Journal Steroid Biochemistry and Molecular Biology. 108:55-63 (2008).
Blum, J. L., Nyagode, B.A., James, M. O, Denslow, N. D. Effects of the pesticide methoxychlor on gene expression in the liver and testes of the male largemouth bass (Micropterus salmoides), Aquatic Toxicology 86:459-69 (2008).
Garcia-Reyero, N., Griffitt, R. J., Liu, L., Kroll, K. J., Farmerie, W. G., Barber, D. S., Denslow, N. D. Construction of a robust microarray from a non-model species (largemouth bass) using pyrosequencing technology. J. Fish Biology, 72: 2354-2376 (2008).
Ongoing Research Support
RO1 ES015449 Denslow (PI) 4/1/06-3/31/09
NIH-NIEHS
Molecular Mechanisms of Endocrine Disruption in Largemouth Bass
The major goals of this research project are to examine the induction of vitellogenin at the molecular level, characterize the estrogen receptors of largemouth bass and to begin to make a DNA array to measure mRNA changes in liver that happen because of estrogen exposure.
Role: PI
US Army Corps of Engineers 7/1/2007-12/31/2008
“Monitoring effects of Endocrine Disrupting Compounds and Munitions on the Fathead Minnow, Pimephales promelas,” The major goal of this research is to test whether exposure to certain munitions will cause endocrine disruption in fathead minnows.
Role: PI |
